A Covalent Enzyme-Substrate Adduct in a Mutant Hen Egg White Lysozyme (D52E)
نویسندگان
چکیده
منابع مشابه
A covalent enzyme-substrate adduct in a mutant hen egg white lysozyme (D52E).
A mutant hen egg white lysozyme, D52E, was designed to correspond to the structure of the mutant T4 lysozyme T26E (Kuroki, R., Weaver, L. H., and Matthews B. W. (1993) Science 262, 2030-2033) to investigate the role of the catalytic residue on the alpha-side of the saccharide in these enzymes. The D52E mutant forms a covalent enzyme-substrate adduct, which was detected by electron ion spray mas...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1997
ISSN: 0021-9258
DOI: 10.1074/jbc.272.32.19976